melting curve analysis (Roche)

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Roche melting curve analysis
Melting Curve Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/melting+curve+analysis/LightCycler+480+System/pm41519330-119-0-12
Average 99 stars, based on 6 article reviews

melting curve analysis - by Bioz Stars, 2026-09

99/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: The effect of ApoE e4 on blood pressure in patients with and without depression
Article Snippet: .. Genotyping was performed by real-time polymerase chain reaction with allelespecific fluorescence energy transfer probes and melting curve analysis on the LightCycler system (Roche Diagnostics, Basel, Switzerland). .. DNA was extracted from 300 μL of whole blood using MagNA Pure LC DNA Isolation Kit – Large Volume on the MagNA Pure LC (Roche Diagnostics), eluted and diluted to 1 mL, of which 5 μL was used in each assay.

Article Title: Protein engineering and in silico approaches to enhance bacterial lactase activity: A global perspective.
Article Snippet: SYPRO Orange® dye (Sigma Aldrich) was added at a final 10× concentration to protein samples at 2 μM in 20 mM Tris-HCl buffer (pH 7.4) containing 50 mM NaCl. .. Melting-curve analysis was performed on a LightCycler 480 II real-time PCR system (Roche) with continuous data acquisition during a temperature ramp from 37 °C to 95 °C. ..

Article Title: The sodium/iodide symporter NIS is a transcriptional target of the p53-family members in liver cancer cells.
Article Snippet: .. Real-time PCR and melting curve analysis were done in a LightCycler 3.0 (Roche Diagnostics) using SYBR Green master mix (Roche Diagnostics). .. The following primers were purchased from (Invitrogen): NIS, forward (fwd) (50-CTTCTGAACTCGGTCCTCACAC-30) and reverse (rev) (50-TCCAGAATGTATAGCGGCTC-30).

Article Title: Pathogenicity and toxin production of different <i>Fusarium oxysporum</i> isolates infecting onion ( <i>Allium cepa</i> L.)
Article Snippet: Funding information Maiju ja Yrjö Rikalan Puutarhasäätiö; Ministry of Agriculture and Forestry of Finland, Grant/ Award Number: 2222/03.01.02/2015 Abstract Fusarium oxysporum forma specialis cepae strains are pathogens causing Fusarium basal rot of onion, which is a major problem in onion cultivation worldwide.. In this work, the pathogenicity and toxin production of different F. oxysporum isolates, originating either from mature harvested onions or from onion sets, were studied.. The F. oxysporum isolates belonging to the f. sp. cepae phylogenetic clade had the full set of seven secreted in xylem (SIX) genes and the gene C5, whereas all the other F. oxysporum isolates tested were negative for these virulence-related genes.

Article Title: Aspartate aminotransferase to platelet ratio index and sustained virologic response are associated with progression from hepatitis C associated liver cirrhosis to hepatocellular carcinoma after treatment with pegylated interferon plus ribavirin
Article Snippet: .. Serum HCV RNA was quantified at baseline and 24 weeks posttreatment using real-time polymerase chain reaction with a detection limitation of 15 IU/mL.26 The threshold for discriminating low- and high-baseline HCV RNA was 400,000 IU/mL.22 HCV genotyping was performed using melting curve analysis (Roche LightCycler; Biotronics Tech Corp., Lowell, MA, USA).27 sustained virologic response SVR was defined as an undetectable HCV RNA for at least 24 weeks after the patient completed the combined treatment of PEG-IFN plus RBV. .. Patients who were positive for HCV RNA at week 24 posttreatment were considered non-SVR. statistical analysis SPSS 19.0 for Windows (IBM Corporation, Armonk, NY, USA) was used for all statistical analyses.

Fluorescence:

Mutagenesis:

Article Title: Hearing in Schoolchildren After Neonatal Exposure to a High-Dose Gentamicin Regimen.
Article Snippet: DNA was extracted by using the Quick-DNA Urine Kit (Zymo Research, Irvine, CA). .. The m.1555A.G gene mutation was analyzed by using polymerase chain reaction amplification and melting curve analysis (LightCycler 480; Roche, Basel, Switzerland). .. Pure tone audiometry thresholds were measured with the Equinox 2.0 clinical audiometer by using Equinox Suite 2.9.0 software (Interacoustics A/S, Middelfart, Denmark).

Polymerase Chain Reaction:

Article Title: Real-Time PCR to Detect α-1 Antitrypsin S and Z Alleles in Formalin-Fixed Paraffin-Embedded Tissue.
Article Snippet: .. PCR amplification and melting curve analysis were performed sequentially in the LightCycler 2.0 instrument (Roche). ..

Amplification:

SYBR Green Assay:

other:

Article Title: ISMD2014Tenth International Symposium on Molecular Diagnostics
Article Snippet: The LCT C/T-13910 polymorphism was performed using a melting curve analysis on the LightCycler Instrument (Roche Diagnostics, Rotkreuz, Switzerland).

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